Journal: PLoS ONE

Article Title: Adaptation of Saccharomyces cerevisiae Cells to High Ethanol Concentration and Changes in Fatty Acid Composition of Membrane and Cell Size

doi: 10.1371/journal.pone.0002623

Figure Lengend Snippet: Ethanol-adapted strain series were cultivated in YPD medium in the presence of 10% ethanol and the fatty acid composition of each strain was determined. Fatty acid compositions of FY834 strain cultured in the presence and absence of ethanol were also measured. Data represented the average values of three independent experiments with standard deviations.

Article Snippet: Yeast cells were precultured in 5 ml of YPD medium (1% Bacto yeast extract, 2% Bacto peptone, 2% glucose) in a test tube at 30°C for 24 h. Then, for the main culture, the precultured yeast was cultivated aerobically at 30°C in a Sakaguchi flask containing 100 ml of YPD medium.

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Adaptation of Saccharomyces cerevisiae Cells to High Ethanol Concentration and Changes in Fatty Acid Composition of Membrane and Cell Size

doi: 10.1371/journal.pone.0002623

Figure Lengend Snippet: The yeast cells obtained through the repetitive cultivation with a stepwise increase in the ethanol concentration were cultivated in the YPD medium and the cell morphology was observed. (A) Microscopic observation of cell morphologies of ethanol-adapted strain series A4 and non-adapted yeast strain (FY834). The bars in each photograph represent 25 µm. (B) Comparison of cell size between non-adapted strain and adapted strain series at mid-log phase. ML, the longest diameter of mother cell; MS, the shortest diameter of mother cell; DL, the longest diameter of daughter cell; DS, the shortest diameter of daughter cell. Data represent the average size of counted 50 cells with standard deviations.

Article Snippet: Yeast cells were precultured in 5 ml of YPD medium (1% Bacto yeast extract, 2% Bacto peptone, 2% glucose) in a test tube at 30°C for 24 h. Then, for the main culture, the precultured yeast was cultivated aerobically at 30°C in a Sakaguchi flask containing 100 ml of YPD medium.

Techniques: Concentration Assay, Comparison